Extracting Onion Dna - 1346 Words

Extraction of DNA from an Onion Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants. DNA was discovered in the 1950’s, there still remains a lot to be known about it, especially how it is used to determine the physical traits that we all have, and how it regulates the workings of the body. deoxyribonucleic acid is a chemical, we can do reactions with it just like we can work with any other chemical. Experiment: Note: You should write all observations from this lab in the observation section on the third page of this lab. These observations will account for a large part of your grade, so be neat and complete! 1) Prepare a buffer solution by pouring the†¦show more content†¦The (hopefully) clear solution you have in this test tube consists of dissolved DNA fragments, as well as some other biochemical compounds such as RNA and some proteins. DNA is a very long molecule, but compared to the holes in a piece of filtering paper, the molecule is still small enough to pass through. 7) Obtain some ice-cold rubbing alcohol from an ice bath or a freezer. Using a drinking straw, gently add rubbing alcohol to the top until there is about an inch and a half sitting above the buffer solution. The best way to do this is to dip the drinking straw into the isopropanol bottle and then when it has filled to put your finger over the end. To add it to the test tube, slowly let it run down the side of the test tube into the DNA solution. Your goal is to have the rubbing alcohol stay on the top of the DNA solution, with as little mixing as possible. The rubbing alcohol is used to extract the DNA from the onion juice. The reason you want the rubbing alcohol to stay on top of the onion juice is because by doing that the liquid will form two distinct layers. Generally, molecules are attracted to the boundaries of two liquids - sometimes the concentration of large molecules can be much higher at the boundary between two liquids. That’s what we’re hoping for here... if the DNA is attracted to the surface, we can pull most of it out. However, if the alcohol and onion juice mixes too much, there will be too much alcohol throughoutShow MoreRelatedExtraction of DNA from onions1450 Words   |  6 PagesEXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes, DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate, namely: Dische Test, Murexide Test, Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleicRead MoreShould New Zealand Genetically Modify Crops For Economic Purposes? Essay4171 Words   |  17 PagesGenetic modification has been used in New Zealand since the 1970’s. Genetic modification, also known as Genetic Engineering is the act of directly manipulating the characteristics of an organism by modifying its genetic materials (Deoxyribonucleic acid, DNA) to produce new or improved organisms. In 1996 New Zealand passed the ‘Hazardous Substances and New Organisms’ act which regulates the research and the release of anything that did not already exist in New Zealand. The act includes organisms thatRead MoreCloning Should Not Be Legalized2450 Words   |  10 PagesCloning Should Not be Legalized Human cloning has been a very controversial issue for a number of years. In order to understand cloning better is important to recognize what cloning is. A clone is an exact genetic replica of a DNA molecule, cell, tissue, organ, or entire plant or animal. This may be done through reproductive cloning or therapeutic cloning known as stem cell cloning. (Coalition for the Advancement of Medical Research) Human cloning raises the issues of whether or not a cloned personRead MoreNatural Dyes11205 Words   |  45 Pagesdyes from one-seeded juniper, Juniperus monosperma, Navajo tea, Thelesperma gracile,[28] or alder bark.[29] [edit] Yellows Yellow dyes are about as numerous as red ones,[30] and can be extracted from saffron, pomegranate rind, turmeric, safflower, onion skins, and a number of weedy flowering plants.[30][31] There is limited evidence of the use of weld (Reseda luteola), also called mignonette or dyers rocket [32] before the Iron Age,[30] but it was an important dye of the ancient Mediterranean andRead MoreSexually Transmitted Diseases35655 Words   |  143 PagesPhysical Environment Weather and Climate 100-113 Soils 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on population

Enta Free Essays

Are entrepreneurs born or created? New Zealand needs more successful entrepreneurs and much thought has been given to how that may be encouraged. C an we create entrepreneurs? Is the ability to see a business opportunity and take the risks necessary to exploit it endowed by nature or is it a result of nurture? I had always thought it was nurture. But recently I learnt more about my own family history and it made me question whether genetics has a bigger influence than I thought. We will write a custom essay sample on Enta or any similar topic only for you Order Now My Hillock ancestors can be traced back eight generations to Edward Hillock who was born in 1687 and lived in the small Essex village of Shades. He was a carpenter and commercial property owner (leasing out the land and building occupied by one of the village’s many public houses). That combination of being self-employed and owning property has since been shared by eight generations of Hillock men spread over more than 300 years. During that time there have been carpenters, farmers, millers, a brewer (Hillocks Karol Ale was popular in the sass) ND a pioneer developer of New Zealand farmland. Perhaps something in our shared genes encouraged this strong legacy of enterprise? To find out more I read Danish writer Lone Franks 2012 book My Beautiful Genome. She summarizes the 52 NOVEMBER 2013 latest research on how our genes influence our health and behavior. Among many issues, she discusses the L variant of the MAO gene, which has been linked with aggression. This variant is known as the â€Å"warrior gene† and is carried by an unusually high proportion of male prison inmates. Most men who carry the warrior gene are not, however, involved in crime. It seems to influence different people in different ways. Carriers of the MAO-L gene appear more willing to take risks in general while also being better at assessing their chance of success in high-pressure situations. That’s useful in battle but the gene also seems to influence characteristics that favor entrepreneurship. A California Institute of Technology study in 2010 tested this possibility in a financial simulation. The 83 men tested had to choose between a safe option and various other options which offered higher risks along with a range of identically higher returns. .. It seems more likely that a shared family culture and perhaps resources handed down from one generation to the next is what sustains these family traditions of business success The men carrying the MAO-L gene were much more likely to take financial risks but only where the odds were in their favor – they were good at assessing risk while also being prepared to take on the risk to generate the return. Those men who did not carry the MAO-L gene typically chose the lower risk and lower return option and were tryingly averse to any potential downside. This research shows there could be a genetic influence on entrepreneurship – at least in Menlo . The warrior gene (perhaps in time it will also be called the â€Å"entrepreneur gene†) cannot, however, explain multiple generations of fathers and sons starting and running businesses. That’s because the MAO gene is linked to the X-chromosome. Men inherit their single copy of the gene from their mother, not their father. Around one-third of all men of European descent carry the MAO-L variant so there is still a good chance that several f my male ancestors carried it, or that I carry it today. With the rapid advances being made in genetic research it is possible that some other gene will be linked with entrepreneurship and help explain why some families (including the Hillocks) have such strong records of entrepreneurship. But unless that occurs it seems more likely that a shared family culture and perhaps resources handed down from one generation to the next is what sustains these family traditions of business success. Lone Frank explains that the expression of many genes is influenced by such environmental factors. It appears that aggressive outcomes for those carrying the MAO-L gene are triggered by conditions such as mothers who smoke or drink during pregnancy or childhood trauma. In a different and more positive environment the gene variant could influence business success. Such an environment would of course also encourage success in those without the MAO-L gene. While there is evidence that some people have greater natural affinity for entrepreneurship it is likely that it is the environment in which people live or work that has the greatest influence. For now we can’t alter our genes but we can create the environment in which more people are encouraged to set up new businesses and be more likely to succeed. . I am not aware of any similar research done on women who carry the MAO-L variant John Hillock is Director at Absolute Certainty Ltd. He can be contacted at john@absolute-certainty. Com Plan your success in the CA Program with our comprehensive PREP Workshops and Exam Coaching dad Leaders in accounting education Pass your exams and become a chartered accountant in 2014! See preparers. Co. NZ Daniel hunt How to cite Enta, Papers

Environmental Protection Agency

Question: Discuss about the . Answer: Introduction The Volkswagen emissions scandaltook place on 18th September 2015, at the time when the notice of violation of Clean Air Act was issued by United States Environmental Protection Agency(EPA). It had been identified that the company has carried out intentional programmed turbocharged direct injection(TDI)diesel engines so that some emission control could be activated during the emission test in the laboratory (Volkswagen, 2016). Discussion Volkswagen has been bluffing the emission test by making their cars appear less polluting than they actually are. The case of the Volkswagen emission scandal is considered as failure to the business ethics as the company has been accused by the EPA for modifying the software on the 3 litre diesel engines fitted to some of the cars. The carbon dioxide emission level test found irregularities in these cars. The company became the target of the regulatory investigation in a number of countries and the stock price of the company fell drastically as a result of the scandal (Sloat, 2016). The Volkswagen emission scandal is a violation against the business ethics as the company created a negative externality for its own profit. As per the report, about 11 million cars of the company were involved in the falsified emission report. The scandal not only created a huge loss for the company but had affected the economy significantly. The excess pollution emitted by the cars resulted in a number of deaths and other non fatal health impacts and environmental consequences. Conclusion The unethical behaviour of Volkswagen was widespread and was a complete failure in terms of the Corporate Social Responsibility (CSR). The company considered that it hardly made any difference if the cars produced by the company adversely affected the environment by emitting 40 times the legal limit of the nitrogen dioxide emission. The company tried thus tried to become one of the leading car manufacturer in the world through unethical manner. References Sloat, W. (2016).Volkswagen Emissions Scandal Relates to 11 Million Cars.WSJ. Retrieved 13 November 2016, from https://www.wsj.com/articles/volkswagen-emissions-scandal-relates-to-11-million-cars-1442916906 Volkswagen,. (2016).Volkswagen Business Ethics and Bad Behavior - What now?.Chuck Gallagher. Retrieved 13 November 2016, from https://www.chuckgallagher.com/volkswagen-business-ethics-and-bad-behavior-what-now/